Difference between revisions of "Nelson:To Do - Sid"

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m (The List)
m (The List)
Line 15: Line 15:
 
*transform RNAp into freshly made competent cells
 
*transform RNAp into freshly made competent cells
 
*spin down the 6L of cells found in cold room
 
*spin down the 6L of cells found in cold room
 +
*put away dishes
 
*prepare DEAE cellulose according to instructions (look for it on protocols page)
 
*prepare DEAE cellulose according to instructions (look for it on protocols page)
 
*make tet-LB plates <br>
 
*make tet-LB plates <br>
 
*streak Xl1-blue onto tet-LB plate <br>
 
*streak Xl1-blue onto tet-LB plate <br>
 
*prepare top-agar <br>
 
*prepare top-agar <br>

Revision as of 07:26, 7 April 2009

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The List

  • prepare CaCl2 competent Rosetta pLys cells
  • transform RNAp into freshly made competent cells
  • spin down the 6L of cells found in cold room
  • put away dishes
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar