Nelson:To Do - Sid: Difference between revisions
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*transform RNAp into freshly made competent cells | *transform RNAp into freshly made competent cells | ||
*spin down the 6L of cells found in cold room | *spin down the 6L of cells found in cold room | ||
*put away dishes | |||
*prepare DEAE cellulose according to instructions (look for it on protocols page) | *prepare DEAE cellulose according to instructions (look for it on protocols page) | ||
*make tet-LB plates <br> | *make tet-LB plates <br> | ||
*streak Xl1-blue onto tet-LB plate <br> | *streak Xl1-blue onto tet-LB plate <br> | ||
*prepare top-agar <br> | *prepare top-agar <br> | ||
Revision as of 07:26, 7 April 2009
The List
- prepare CaCl2 competent Rosetta pLys cells
- transform RNAp into freshly made competent cells
- spin down the 6L of cells found in cold room
- put away dishes
- prepare DEAE cellulose according to instructions (look for it on protocols page)
- make tet-LB plates
- streak Xl1-blue onto tet-LB plate
- prepare top-agar
