Difference between revisions of "Nelson:To Do - Sid"

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==The List==
 
==The List==
*prepare CaCl2 competent Rosetta pLys cells
+
*prepare CaCl<sub>2</sub> competent Rosetta pLys cells
 
*transform RNAp into freshly made competent cells
 
*transform RNAp into freshly made competent cells
 
*spin down the 6L of cells found in cold room
 
*spin down the 6L of cells found in cold room

Revision as of 06:31, 7 April 2009

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The List

  • prepare CaCl2 competent Rosetta pLys cells
  • transform RNAp into freshly made competent cells
  • spin down the 6L of cells found in cold room
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar