Difference between revisions of "Nelson:To Do - Eric"

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m (The List)
m (The List)
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==The List==
 
==The List==
  
 
+
*dishes
 
*pour 2 SDS-PAGE gels
 
*pour 2 SDS-PAGE gels
 
*prepare DEAE cellulose according to instructions (look for it on protocols page)
 
*prepare DEAE cellulose according to instructions (look for it on protocols page)

Revision as of 10:48, 6 April 2009

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The List

  • dishes
  • pour 2 SDS-PAGE gels
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make 1 L of 1 M Tris-HCl, pH 8.0
  • 6L of LB and autoclave
  • transform polg-a into Rosetta cells
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar
  • monitor cells (your mutant) in shaker and turn down to 15 deg. C when OD reaches about 0.5