Nelson:To Do - Eric: Difference between revisions
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*transform your mutant into Rosetta2 cells | *transform your mutant into Rosetta2 cells | ||
*transform TFAM into Rosetta2 cells | |||
*pour 1 or 2 SDS-PAGE gels | |||
*prepare DEAE cellulose according to instructions (look for it on protocols page) | *prepare DEAE cellulose according to instructions (look for it on protocols page) | ||
Revision as of 07:12, 3 April 2009
The List
- transform your mutant into Rosetta2 cells
- transform TFAM into Rosetta2 cells
- pour 1 or 2 SDS-PAGE gels
- prepare DEAE cellulose according to instructions (look for it on protocols page)
- make 1 L of 1 M Tris-HCl, pH 8.0
- wash out 2 L flasks and make 6L of LB and autoclave
- make tet-LB plates
- streak Xl1-blue onto tet-LB plate
- prepare top-agar
