Difference between revisions of "NanoBio: Antibiotics"

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Line 7: Line 7:
 
!Stock conc. (mg/mL)
 
!Stock conc. (mg/mL)
 
!Final conc. (ug/mL)
 
!Final conc. (ug/mL)
 +
!Tube/Plate color
 
|-
 
|-
 
|ampillicin
 
|ampillicin
Line 12: Line 13:
 
|100
 
|100
 
|100
 
|100
 +
|blue
 
|-
 
|-
 
|chloramphenicol
 
|chloramphenicol
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|30
 
|30
 
|30
 
|30
 +
|red
 
|-
 
|-
 
|kanamycin
 
|kanamycin
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|50
 
|50
 
|50
 
|50
 +
|green
 
|-
 
|-
 
|tetracycline
 
|tetracycline
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|12
 
|12
 
|12
 
|12
 +
|purple
 
|-
 
|-
 
|}
 
|}

Revision as of 12:11, 6 February 2013

Antibiotic Concentrations for Plates & Liquid Media

  • Note that these concentrations are significantly higher than those recommended in "Molecular Cloning."
Antibiotic solvent Stock conc. (mg/mL) Final conc. (ug/mL) Tube/Plate color
ampillicin water 100 100 blue
chloramphenicol methanol 30 30 red
kanamycin water 50 50 green
tetracycline 70% ethanol 12 12 purple

return to Protocols

CAjoF 14:48, 21 December 2007 (CST):