NanoBio:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
Arthur K Yu (talk | contribs) (added Agarose Gels!) |
||
| Line 20: | Line 20: | ||
*[[NanoBio: Bacterial Transformation | Bacterial Transformation]] | *[[NanoBio: Bacterial Transformation | Bacterial Transformation]] | ||
*[[NanoBio: Plasmid Verification | Plasmid Verification]] | *[[NanoBio: Plasmid Verification | Plasmid Verification]] | ||
*[[NanoBio: AgaroseGels | Agarose Gels]] | |||
==Protein Expression & Purification == | ==Protein Expression & Purification == | ||
Revision as of 11:41, 17 June 2008
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- Installing Vector NTI How to install Vector NTI, a free sequence editor
- BioBrick & Biofusion Strategies for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- 3 Antibiotic Assembly
- Bacterial Transformation
- Plasmid Verification
- Agarose Gels
Protein Expression & Purification
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
