NanoBio:Protocols

Nano Synth Bio

General Molecular Biology

Current Protocols in Molecular Biology

Lab Practices

• Keeping a Laboratory Notebook

Sub-cloning

• Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
• Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
• Installing Vector NTI How to install Vector NTI, a free sequence editor
• BioBrick & Biofusion Strategies for Making Parts An overview of how to make & combine parts.
• 3 Antibiotic Assembly
• Commonly Used Plasmids
• Commonly Used Primers
• PCR
• Oligonucleotide Inserts
• Overlapping Oligonucleotide Inserts
• Site-Directed Mutagenesis
• Restriction Digest
• Ligation
• Bacterial Transformation of Chemically Competent Cells
• Prep for Electrocompetent cells
• Bacterial Transformation using Electroporation
• Plasmid Verification
• Archiving Your Plasmid in the Strain Collection
• Agarose Gels

Protein Expression & Purification

• Bacterial Hosts for Overexpression
• Test Induction & Purification
• Protein Purification
• Isolation of Periplasmic Fraction
• Protein Gels
• Cell Surface Binding Assay

Gene deletion with λ-red

• Overview and Materials for λ-red
• Primer Design
• Protcol for gene knockout
• Knockout/in

Genomic Integration into S. cerevisiae

• Integration into Yeast
• Verifying Genomic Integration

Lipid Bilayers

• Making Unilamellar Vesicles
• Preparation of Supported Bilayers
• Cleaning SiO2 surfaces

Care of Synechocystis sp 6803

• Liquid & Solid Growth Media
• Guidelines from 2006 Harvard iGEM team

Primers

• Biobricks & Biofusion Sub-cloning
• Synechocystis sp. 6803
• Shewanella oneidensis MR-1
• Guidelines on Talking or Writing about your project
• Microbial Respiration

Recipes

• LB media & plates
• Antibiotics
• TSB media
• BG-11 media
• BG-11 plates
• Supplemented M9 Media

Safety & Waste Disposal

• Safety & Waste Disposal