Difference between revisions of "NanoBio:Protocols"

From OpenWetWare
Jump to: navigation, search
(Sub-cloning)
(Lab Practices)
 
(7 intermediate revisions by 4 users not shown)
Line 2: Line 2:
 
==General Molecular Biology==
 
==General Molecular Biology==
 
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology]
 
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology]
 +
 +
==Lab Manual==
 +
*[[NanoBio: Notebook| Keeping a Laboratory Notebook]]
  
 
==Sub-cloning ==
 
==Sub-cloning ==
Line 12: Line 15:
 
*[[NanoBio: Commonly Used Primers | Commonly Used Primers]]
 
*[[NanoBio: Commonly Used Primers | Commonly Used Primers]]
 
*[[NanoBio: PCR | PCR]]
 
*[[NanoBio: PCR | PCR]]
 +
*[[NanoBio: Golden Gate Cloning | Golden Gate Cloning]]
 
*[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]]
 
*[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]]
 
*[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]]
 
*[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]]
Line 21: Line 25:
 
*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]]
 
*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]]
 
*[[NanoBio: Plasmid Verification | Plasmid Verification]]
 
*[[NanoBio: Plasmid Verification | Plasmid Verification]]
 +
*[[NanoBio: Archiving Your Plasmid in the Strain Collection | Archiving Your Plasmid in the Strain Collection]]
 
*[[NanoBio: AgaroseGels | Agarose Gels]]
 
*[[NanoBio: AgaroseGels | Agarose Gels]]
  
Line 27: Line 32:
 
*[[NanoBio: Test Induction & Purification|Test Induction & Purification]]
 
*[[NanoBio: Test Induction & Purification|Test Induction & Purification]]
 
*[[NanoBio: Protein Purification|Protein Purification]]
 
*[[NanoBio: Protein Purification|Protein Purification]]
 +
*[[NanoBio: Isolation of Periplasmic Fraction|Isolation of Periplasmic Fraction]]
 
*[[NanoBio: Protein Gels|Protein Gels]]
 
*[[NanoBio: Protein Gels|Protein Gels]]
 
*[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]]
 
*[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]]
Line 34: Line 40:
 
*[[NanoBio: Primer Design|Primer Design]]
 
*[[NanoBio: Primer Design|Primer Design]]
 
*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]]
 
*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]]
 +
*[[NanoBio: Knockout/in|Knockout/in]]
  
 
==Genomic Integration into S. cerevisiae==
 
==Genomic Integration into S. cerevisiae==
Line 42: Line 49:
 
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]]
 
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]]
 
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]]
 
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]]
 +
*[[NanoBio: Cleaning SiO2 surfaces| Cleaning SiO2 surfaces]]
 +
 
==Care of Synechocystis sp 6803==
 
==Care of Synechocystis sp 6803==
 
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]]
 
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]]
Line 60: Line 69:
 
* [[NanoBio: BG-11 plates| BG-11 plates]]
 
* [[NanoBio: BG-11 plates| BG-11 plates]]
 
* [[NanoBio: Supplemented M9 Media| Supplemented M9 Media]]
 
* [[NanoBio: Supplemented M9 Media| Supplemented M9 Media]]
 +
* [[NanoBio: Stock Solutions | Stock Solutions]]
  
 
==Safety & Waste Disposal==
 
==Safety & Waste Disposal==
 
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]]
 
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]]

Latest revision as of 16:12, 1 May 2013

Nano Synth Bio

General Molecular Biology

Current Protocols in Molecular Biology

Lab Manual

Sub-cloning

Protein Expression & Purification

Gene deletion with λ-red

Genomic Integration into S. cerevisiae

Lipid Bilayers

Care of Synechocystis sp 6803

Primers

Recipes

Safety & Waste Disposal