NanoBio:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Arthur K Yu (talk | contribs) |
|||
| (22 intermediate revisions by 6 users not shown) | |||
| Line 2: | Line 2: | ||
==General Molecular Biology== | ==General Molecular Biology== | ||
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] | [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] | ||
==Lab Manual== | |||
*[[NanoBio: Notebook| Keeping a Laboratory Notebook]] | |||
==Sub-cloning == | ==Sub-cloning == | ||
| Line 7: | Line 10: | ||
*[[Arking:JCATutorialIntro1| Introduction to BioBricks]] A nice introduction to BioBrick cloning, written by Chris Anderson | *[[Arking:JCATutorialIntro1| Introduction to BioBricks]] A nice introduction to BioBrick cloning, written by Chris Anderson | ||
*[[NanoBio: Installing Vector NTI| Installing Vector NTI]] How to install Vector NTI, a free sequence editor | *[[NanoBio: Installing Vector NTI| Installing Vector NTI]] How to install Vector NTI, a free sequence editor | ||
*[[NanoBio: Biobrick/fusion Strategy | BioBrick & Biofusion Strategies for Making Parts]] | *[[NanoBio: Biobrick/fusion Strategy | BioBrick & Biofusion Strategies for Making Parts]] An overview of how to make & combine parts. | ||
*[[ | *[[Synthetic_Biology:BioBricks/3A_assembly| 3 Antibiotic Assembly]] | ||
*[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]] | *[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]] | ||
*[[NanoBio: Commonly Used Primers | Commonly Used Primers]] | *[[NanoBio: Commonly Used Primers | Commonly Used Primers]] | ||
*[[NanoBio: PCR | PCR]] | *[[NanoBio: PCR | PCR]] | ||
*[[NanoBio: Golden Gate Cloning | Golden Gate Cloning]] | |||
*[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]] | *[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]] | ||
*[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]] | *[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]] | ||
| Line 17: | Line 21: | ||
*[[NanoBio: Restriction Digest | Restriction Digest]] | *[[NanoBio: Restriction Digest | Restriction Digest]] | ||
*[[NanoBio: Ligation | Ligation]] | *[[NanoBio: Ligation | Ligation]] | ||
*[[ | *[[NanoBio: Bacterial Transformation | Bacterial Transformation of Chemically Competent Cells]] | ||
*[[NanoBio: Bacterial Transformation | Bacterial Transformation]] | *[[NanoBio: Prep for Electrocompetent cells | Prep for Electrocompetent cells]] | ||
*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]] | |||
*[[NanoBio: Plasmid Verification | Plasmid Verification]] | *[[NanoBio: Plasmid Verification | Plasmid Verification]] | ||
* | *[[NanoBio: Archiving Your Plasmid in the Strain Collection | Archiving Your Plasmid in the Strain Collection]] | ||
*[[NanoBio: AgaroseGels | Agarose Gels]] | |||
==Protein Expression & Purification == | |||
*[[NanoBio: Bacterial Hosts for Overexpression|Bacterial Hosts for Overexpression]] | |||
*[[NanoBio: Test Induction & Purification|Test Induction & Purification]] | |||
*[[NanoBio: Protein Purification|Protein Purification]] | |||
*[[NanoBio: Isolation of Periplasmic Fraction|Isolation of Periplasmic Fraction]] | |||
*[[NanoBio: Protein Gels|Protein Gels]] | |||
*[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]] | |||
==Gene deletion with λ-red == | |||
*[[NanoBio: Overview and Materials for λ-red|Overview and Materials for λ-red]] | |||
*[[NanoBio: Primer Design|Primer Design]] | |||
*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]] | |||
*[[NanoBio: Knockout/in|Knockout/in]] | |||
==Genomic Integration into S. cerevisiae== | ==Genomic Integration into S. cerevisiae== | ||
| Line 29: | Line 49: | ||
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]] | *[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]] | ||
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]] | *[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]] | ||
*[[NanoBio: Cleaning SiO2 surfaces| Cleaning SiO2 surfaces]] | |||
==Care of Synechocystis sp 6803== | ==Care of Synechocystis sp 6803== | ||
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]] | *[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]] | ||
| Line 46: | Line 68: | ||
* [[NanoBio: BG-11 media | BG-11 media]] | * [[NanoBio: BG-11 media | BG-11 media]] | ||
* [[NanoBio: BG-11 plates| BG-11 plates]] | * [[NanoBio: BG-11 plates| BG-11 plates]] | ||
* [[NanoBio: Supplemented M9 Media| Supplemented M9 Media]] | |||
* [[NanoBio: Stock Solutions | Stock Solutions]] | |||
==Safety & Waste Disposal== | ==Safety & Waste Disposal== | ||
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]] | *[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]] | ||
Latest revision as of 16:12, 1 May 2013
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Lab Manual
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- Installing Vector NTI How to install Vector NTI, a free sequence editor
- BioBrick & Biofusion Strategies for Making Parts An overview of how to make & combine parts.
- 3 Antibiotic Assembly
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Golden Gate Cloning
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Bacterial Transformation of Chemically Competent Cells
- Prep for Electrocompetent cells
- Bacterial Transformation using Electroporation
- Plasmid Verification
- Archiving Your Plasmid in the Strain Collection
- Agarose Gels
Protein Expression & Purification
- Bacterial Hosts for Overexpression
- Test Induction & Purification
- Protein Purification
- Isolation of Periplasmic Fraction
- Protein Gels
- Cell Surface Binding Assay
Gene deletion with λ-red
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
Recipes
- LB media & plates
- Antibiotics
- TSB media
- BG-11 media
- BG-11 plates
- Supplemented M9 Media
- Stock Solutions
