Difference between revisions of "NanoBio:Protocols"

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==General Molecular Biology==
 
==General Molecular Biology==
 
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology]
 
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology]
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==Lab Practices==
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*[[NanoBio: Notebook| Keeping a Laboratory Notebook]]
  
 
==Sub-cloning ==
 
==Sub-cloning ==
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*[[NanoBio: Ligation | Ligation]]
 
*[[NanoBio: Ligation | Ligation]]
 
*[[NanoBio: Bacterial Transformation | Bacterial Transformation of Chemically Competent Cells]]
 
*[[NanoBio: Bacterial Transformation | Bacterial Transformation of Chemically Competent Cells]]
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*[[NanoBio: Prep for Electrocompetent cells | Prep for Electrocompetent cells]]
 
*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]]
 
*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]]
 
*[[NanoBio: Plasmid Verification | Plasmid Verification]]
 
*[[NanoBio: Plasmid Verification | Plasmid Verification]]
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*[[NanoBio: Archiving Your Plasmid in the Strain Collection | Archiving Your Plasmid in the Strain Collection]]
 
*[[NanoBio: AgaroseGels | Agarose Gels]]
 
*[[NanoBio: AgaroseGels | Agarose Gels]]
  
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*[[NanoBio: Test Induction & Purification|Test Induction & Purification]]
 
*[[NanoBio: Test Induction & Purification|Test Induction & Purification]]
 
*[[NanoBio: Protein Purification|Protein Purification]]
 
*[[NanoBio: Protein Purification|Protein Purification]]
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*[[NanoBio: Isolation of Periplasmic Fraction|Isolation of Periplasmic Fraction]]
 
*[[NanoBio: Protein Gels|Protein Gels]]
 
*[[NanoBio: Protein Gels|Protein Gels]]
 
*[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]]
 
*[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]]
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==Gene deletion with λ-red ==
 
==Gene deletion with λ-red ==
 
*[[NanoBio: Overview and Materials for λ-red|Overview and Materials for λ-red]]
 
*[[NanoBio: Overview and Materials for λ-red|Overview and Materials for λ-red]]
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*[[NanoBio: Primer Design|Primer Design]]
 
*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]]
 
*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]]
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*[[NanoBio: Knockout/in|Knockout/in]]
  
 
==Genomic Integration into S. cerevisiae==
 
==Genomic Integration into S. cerevisiae==
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*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]]
 
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]]
 
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]]
 
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]]
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*[[NanoBio: Cleaning SiO2 surfaces| Cleaning SiO2 surfaces]]
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==Care of Synechocystis sp 6803==
 
==Care of Synechocystis sp 6803==
 
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]]
 
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]]

Revision as of 12:53, 21 March 2013

Nano Synth Bio

General Molecular Biology

Current Protocols in Molecular Biology

Lab Practices

Sub-cloning

Protein Expression & Purification

Gene deletion with λ-red

Genomic Integration into S. cerevisiae

Lipid Bilayers

Care of Synechocystis sp 6803

Primers

Recipes

Safety & Waste Disposal