NanoBio:Protocols: Difference between revisions
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==Sub-cloning == | ==Sub-cloning == | ||
* | *[http://www.openwetware.org/wiki/Arking:JCATutorialIntro7| Introduction to Cloning] A nice introduction to cloning, written by Chris Anderson | ||
* | *[http://www.openwetware.org/wiki/Arking:JCATutorialIntro1| Introduction to BioBricks] A nice introduction to BioBrick cloning, written by Chris Anderson | ||
*[[NanoBio: Biobrick/fusion Strategy | BioBrick & Biofusion Strategies for Making Parts]] | *[[NanoBio: Biobrick/fusion Strategy | BioBrick & Biofusion Strategies for Making Parts]] | ||
*[[NanoBio: Designing Parts or Genes| Designing Parts or Genes ]] | *[[NanoBio: Designing Parts or Genes| Designing Parts or Genes ]] | ||
Revision as of 17:32, 1 April 2008
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- BioBrick & Biofusion Strategies for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- 3 Antibiotic Assembly
- Bacterial Transformation
- Plasmid Verification
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
