Difference between revisions of "Nachury:Protocols"

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(Protocols)
 
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==Protocols==
 
==Protocols==
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=<center><font color="red">Under (very slow) construction</font></center>=
  
===Extracts===
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===Cell Lines===
:*[[Nachury:NuclearExtract|Hypotonic/N<sub>2</sub> cavitation lysis]]
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:*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]]
:*[[Nachury:TestisExtract|Hypotonic/Testis extract]]
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:*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]]
:*[[Nachury:TestisExtract|Hypotonic/Retinal extract]]
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:*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]]
:*[[Nachury:TestisExtract|Hypotonic/Retinal detergent extract]]
+
 
 +
===Extractology===
 +
:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]]
 +
:*[[Nachury:Testis_Extract|Testis extract]]
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:*[[Nachury:Retinal_Extract|Retinal extract]]
 +
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]
  
 
===Protein Biochemistry===
 
===Protein Biochemistry===
 +
:*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]]
 
:*[[Nachury:silver|Silver staining]]
 
:*[[Nachury:silver|Silver staining]]
 
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
 
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
 
:*[[Nachury:TCA|TCA precipitation of proteins]]
 
:*[[Nachury:TCA|TCA precipitation of proteins]]
 +
 +
=== Immunofluorescence ===
 +
:*[[Nachury:General|General guidelines]]
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:*[[Nachury:PFA_Saponin|PFA/Saponin]]
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 +
=== Lipids ===
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:*[[Nachury:Liposome_Prep|Preparation of liposomes]]
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:*[[Nachury:Liposome_Binding|Protein-Liposomes interaction assays]]
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:*[[Nachury:PIP_strip|PIP strip blotting assays (fat blot)]]
  
 
===DNA===
 
===DNA===
 
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
 
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
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 +
=== Microtubules ===

Latest revision as of 12:37, 2 November 2011

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Protocols

Under (very slow) construction

Cell Lines

Extractology

Protein Biochemistry

Immunofluorescence

Lipids

DNA

Microtubules