Nachury:Protocols: Difference between revisions
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==Protocols== | ==Protocols== | ||
=<center><font color="red">Under (very slow) construction</font></center>= | |||
===General=== | ===Cell Lines=== | ||
:*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]] | |||
:*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]] | |||
:*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]] | |||
===Extractology=== | |||
:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]] | |||
:*[[Nachury:Testis_Extract|Testis extract]] | |||
:*[[Nachury:Retinal_Extract|Retinal extract]] | |||
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]] | |||
===Protein Biochemistry=== | |||
:*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]] | |||
:*[[Nachury:silver|Silver staining]] | |||
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]] | |||
:*[[Nachury:TCA|TCA precipitation of proteins]] | |||
=== Immunofluorescence === | |||
:*[[Nachury:General|General guidelines]] | |||
:*[[Nachury:PFA_Saponin|PFA/Saponin]] | |||
=== Lipids === | |||
:*[[Nachury:Liposome_Prep|Preparation of liposomes]] | |||
:*[[Nachury:Liposome_Binding|Protein-Liposomes interaction assays]] | |||
:*[[Nachury:PIP_strip|PIP strip blotting assays (fat blot)]] | |||
===DNA=== | |||
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]] | |||
=== Microtubules === | |||
Latest revision as of 12:37, 2 November 2011
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Protocols
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