Difference between revisions of "Nachury:Protocols"

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(Protocols)
(Protocols)
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==Protocols==
 
==Protocols==
 
<center><font color="red">Under construction</font></center>
 
<center><font color="red">Under construction</font></center>
 +
 +
===Cell Lines===
 +
:*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]]
 +
:*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]]
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:*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]]
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===Extractology===
 
===Extractology===
 
:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]]
 
:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]]
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:*[[Nachury:Retinal_Extract|Retinal extract]]
 
:*[[Nachury:Retinal_Extract|Retinal extract]]
 
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]
 
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]
 
  
 
===Protein Biochemistry===
 
===Protein Biochemistry===
 +
:*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]]
 
:*[[Nachury:silver|Silver staining]]
 
:*[[Nachury:silver|Silver staining]]
 
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
 
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]

Revision as of 18:55, 9 January 2008

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Protocols

Under construction

Cell Lines

Extractology

Protein Biochemistry

Immunofluorescence

Lipids

DNA