Muratore:Protocols/Digest/SapI

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SapI digest protocol
Tube sterile H2O 10X NEB4 DNA SapI (2 kU/mL)
plasmid 30 μL 5 μL 10 μL of NheI digest 5μL
insert 30 μL 5 μL 10 μL of NheI digest 5μL
  1. 2h @ 37°C
  2. spin-column purify