Difference between revisions of "Mounting medium"

From OpenWetWare
Jump to: navigation, search
(external links)
(introduction)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
A '''Mounting medium''' is a liquid, polymer, resin, etc, used to mount microscopical specimens before examination. Its most important attributes are refractive index, viscosity, transparency, long-term chemical stability, and preservation of the specimen, esp. its fluorophores.  
+
A '''Mounting medium''' is a liquid, polymer, resin, etc, used to mount microscopical specimens before examination. Its optical attributes (refractive index, transparency) and physical/chemical properties (viscosity, stability, preservation of the specimen) are crucial. Mounting media for fluorescent microscopy often contain anti-fading agents to lengthen the life of fluorophores by quenching free radicals generated during illumination of the specimen.
  
 
=== See also on OWW ===
 
=== See also on OWW ===
Line 8: Line 8:
 
* [[Image:3stars.png]] [http://www.gonda.ucla.edu/bri_core/mounting.htm Mounting medium page by UCLA Brain Institute Imaging Facility]
 
* [[Image:3stars.png]] [http://www.gonda.ucla.edu/bri_core/mounting.htm Mounting medium page by UCLA Brain Institute Imaging Facility]
 
* [[Image:3stars.png]] [http://www.ihcworld.com/_protocols/histology/aqueous_mounting_medium.htm Page with recipes and evaluation of 5 different mounting media by JA Kiernan at IHCWorld]
 
* [[Image:3stars.png]] [http://www.ihcworld.com/_protocols/histology/aqueous_mounting_medium.htm Page with recipes and evaluation of 5 different mounting media by JA Kiernan at IHCWorld]
 +
 +
[[Category:Protocol]]
 +
[[Category:Chemical]]
 +
[[Category:Microscopy]]

Latest revision as of 09:15, 27 April 2010

A Mounting medium is a liquid, polymer, resin, etc, used to mount microscopical specimens before examination. Its optical attributes (refractive index, transparency) and physical/chemical properties (viscosity, stability, preservation of the specimen) are crucial. Mounting media for fluorescent microscopy often contain anti-fading agents to lengthen the life of fluorophores by quenching free radicals generated during illumination of the specimen.

See also on OWW

Related web resources