Mounting medium: Difference between revisions
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A '''Mounting medium''' is a liquid, polymer, resin, etc, used to mount microscopical specimens before examination. Its | A '''Mounting medium''' is a liquid, polymer, resin, etc, used to mount microscopical specimens before examination. Its optical attributes (refractive index, transparency) and physical/chemical properties (viscosity, stability, preservation of the specimen) are crucial. Mounting media for fluorescent microscopy often contain anti-fading agents to lengthen the life of fluorophores by quenching free radicals generated during illumination of the specimen. | ||
=== See also on OWW === | === See also on OWW === | ||
* [[Immunofluorescence Microscopy (Pf, fixed)]] | * [[Immunofluorescence Microscopy (Pf, fixed)]] | ||
* [[Griffin:Immunofluorescence Cell Staining]] | * [[Griffin:Immunofluorescence Cell Staining]] | ||
=== Related web resources === | |||
* [[Image:3stars.png]] [http://www.gonda.ucla.edu/bri_core/mounting.htm Mounting medium page by UCLA Brain Institute Imaging Facility] | |||
* [[Image:3stars.png]] [http://www.ihcworld.com/_protocols/histology/aqueous_mounting_medium.htm Page with recipes and evaluation of 5 different mounting media by JA Kiernan at IHCWorld] | |||
[[Category:Protocol]] | |||
[[Category:Chemical]] | |||
[[Category:Microscopy]] | |||
Latest revision as of 10:15, 27 April 2010
A Mounting medium is a liquid, polymer, resin, etc, used to mount microscopical specimens before examination. Its optical attributes (refractive index, transparency) and physical/chemical properties (viscosity, stability, preservation of the specimen) are crucial. Mounting media for fluorescent microscopy often contain anti-fading agents to lengthen the life of fluorophores by quenching free radicals generated during illumination of the specimen.
