Difference between revisions of "Moore:Protocols"

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[[Sauer:ClpX_purification]]
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===''E. coli'' Strain Construction===
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[[Moore:Working_with_coli|Working With ''E. coli'']]<br>
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[[Sauer:P1vir_phage_transduction|Transducing Genes with P1 Phage]]<br>
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===Cloning and Molecular Biology===
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[[Quantitating_nucleic_acids|Figuring Out How Much Nucleic Acid You Have]]<br>
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[[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br>
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[[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br>
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[[Library_Generation|Making Genomic Libraries]]<br>
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===Protein Purification===
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[[Sauer:Lysing_E._coli_with_Lysozymes|Getting the Cells open]]<br>
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[[Sauer:ClpX_purification|''E. coli'' ClpX]]<br>
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[[Sauer:ClpP_purification/Untagged_ClpP|''E. coli'' ClpP]]<br>
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[[Sauer:SspB_purification|''E. coli'' SspB]]<br>
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===Protein Analysis===
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[[Sauer:Silver_staining|Silver Staining]]<br>
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[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br>
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[[Sauer:Western_blot|Western Blot]]<br>
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[[Moore:Chemiluminescent|Chemiluminescent HRP Detection]]<br>
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===Enymatic Assays===
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[[Beta-Galactosidase_Assay_%28A_better_Miller%29|Miller/β-Galactosidase Assay]]<br>
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===Translation and Ribosomes===
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[[Moore:Ribosome_Purification|''E. coli'' Ribosome Purification]]<br>
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[[Moore:Translation_Extracts|Preparing Translation Extracts]]<br>
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[[Moore:Poly-U|Poly-U Translation]]<br>
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[[Moore:mRNA_Translation|mRNA Translation]]<br>
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===RNA Purification and Analysis===
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[[Sauer:RNA_Purification_from_E._coli|Getting RNA From ''E. coli'']]<br>
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[[Moore:Primer_extension|Primer Extension]]<br>
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Revision as of 08:34, 12 November 2008