Difference between revisions of "Moore:Protocols"

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(RNA Purification and Analysis)
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===''E. coli'' Strain Construction===
[[Moore:Working_with_coli|Working With ''E. coli'']]<br>
[[Sauer:P1vir_phage_transduction|Transducing Genes with P1 Phage}}<br>
===Cloning and Molecular Biology===
===Cloning and Molecular Biology===

Revision as of 08:43, 2 July 2008

E. coli Strain Construction

Working With E. coli
[[Sauer:P1vir_phage_transduction|Transducing Genes with P1 Phage}}

Cloning and Molecular Biology

Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching

Protein Purification

Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB

Protein Analysis

Silver Staining
Western Blot

Translation and Ribosomes

E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation

RNA Purification and Analysis

Getting RNA From E. coli
Primer Extension