Methods to determine the size of an object in microns

From OpenWetWare
Revision as of 09:42, 11 August 2005 by Stpierre (talk | contribs) (=Mathematically)
Jump to: navigation, search

Mathematically

Pixels on our CCD are 6.45um. The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD:

Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}}}

Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us:

60x  : 0.1075 um/pixel or 9.30 pixels/um 40x  : 0.1613 um/pixel or 6.21 pixels/um 20x  : 0.3225 um/pixel or 3.10 pixels/um 10x  : 0.645 um/pixel or 1.55 pixel/um