McClean: Yeast Nomenclature

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Revision as of 08:39, 7 November 2012 by Megan N McClean (talk | contribs) (Alleles created by recombinant DNA technology)
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S. cerevisiae researchers have a systematic approach for describing genotypes of yeast strains. We need to follow this system when writing papers, protocols, and when entering yeast strains into the lab database.

Yeast nomenclature is covered well in the articles listed in the references, so I won't recapitulate all of the details here. You should refer to these references when you have questions. I will just list some of the common conventions that you might need when entering strains into the lab database.


Chromosomal Genes

Nonmendelian Determinants

Genetic Background


Alleles created by recombinant DNA technology

  1. Disruptions
  2. Deletions
    • Deletions are written as the name of the gene that is altered following by -Δ; for example arg2-Δ1, where the number 1 denotes a specific complete or partial deletion of arg2.
  3. Replacements
    • Replacements should be written as the name of the gene that is replaced followed by the symbole Δ::. Examples:
      • If you deleted the entire open reading frame of the GAL1 gene with a drug resistance cassette (such as NatMX for ClonNat resistance) you would write gal1Δ::NatMX.
      • If you deleted the GAl1 open reading frame with a fluorescent reporter (for instance, with GFP-KanMX if you wanted a quick reporter of P\{GAL1} promoter activity) you would write the genotype as ga1Δ::GFP-KanMX


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

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Burke, D. Dawson, D. & Sterns, T. 2000 Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual Cold Spring Harbor Laboratory Press

Sherman, F. 2002 Getting started with yeast Methods Enzymol, 350, 3-41

Saccharomyces cerevisiae Genetic Nomenclature Guide


or instead, discuss this protocol.