McClean: NIS Elements Repair: Difference between revisions

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(New page: <!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! --> ==Overview== On occasion "funny" things happen with the NIS Elements software/drivers. For example, every time you mark a ...)
 
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<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL!  -->
<!-- COPY EVERYTHING BELOW HERE TO START YOUR OWN PROTOCOL!  -->


==Overview==
==Overview==
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==Protocol==
==Protocol==
* Open the Windows ''Start'' menu
* Open the Windows ''Start'' menu
*Choose ''All Programs''
* Choose ''All Programs''
* Choose ''NIS-Elements 3.2 64-bit''
* Choose ''NIS-Elements 3.2 64-bit''
*Choose ''Modify Installation''
* Choose ''Modify Installation''
Click Repair, Next
* Click ''Repair'' and choose ''Next''
* Click ''Next'' or ''Finish'' as necessary to run the utility
* When prompted to restart the computer, do so.


If prompted to restart, restart the computer
==Notes==
==Notes==
<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!-->
<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!-->
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!  Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!  Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
#'''*[[User:Megan N McClean|Megan N McClean]]''' When I can't age my dissection plates on my bench for a few days, I will stick them in the 30°C or 37°C warm room the morning of the day I dissect to dry them out a little bit. It is absolutely infuriating, if not impossible, to try dissection on plates that are wet.
#'''*[[User:Megan N McClean|Megan N McClean]]''' The "stage crashing all the way to the bottom left" error is particularly disturbing as when this happens the stage crashes into whatever objective happens to be in position. If you have any reason to believe that the scope is going to misbehave please remove the objectives before continuing.  A particularly good time to worry about this and remove the objectives before messing with stage positions is if you've been messing with any drivers or installing drivers that might conflict with the NIS elements software.
#'''*[[User:Megan N McClean|Megan N McClean]]''' Different tetrad dissection protocols call for using different enzymes to digest the ascus wall.  Our protocol uses a β-glucuronidase from Sigma (G7770) which is a mixture of enzymes derived from Helix pomatia (the Roman snail).  Zymolyase, another commonly used enzyme, consists mostly of β-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product. β-glucuronidase catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans).
 
==References==
Adapted from Maitreya Dunham's protocol (http://dunham.gs.washington.edu/sporulationdissection.htm) and the Botstein lab protocol (http://www.princeton.edu/genomics/botstein/protocols/Sporulation_and_Tetrad_Dissection.pdf)


==Contact==
==Contact==

Revision as of 12:07, 13 December 2011


Overview

On occasion "funny" things happen with the NIS Elements software/drivers. For example, every time you mark a stage position the stage goes all the way to the left hand bottom corner regardless of what position you marked. When this happens it is time to run the repair utility.

Protocol

  • Open the Windows Start menu
  • Choose All Programs
  • Choose NIS-Elements 3.2 64-bit
  • Choose Modify Installation
  • Click Repair and choose Next
  • Click Next or Finish as necessary to run the utility
  • When prompted to restart the computer, do so.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

  1. *Megan N McClean The "stage crashing all the way to the bottom left" error is particularly disturbing as when this happens the stage crashes into whatever objective happens to be in position. If you have any reason to believe that the scope is going to misbehave please remove the objectives before continuing. A particularly good time to worry about this and remove the objectives before messing with stage positions is if you've been messing with any drivers or installing drivers that might conflict with the NIS elements software.

Contact

or instead, discuss this protocol.


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