McClean: Fixation of Yeast (P. Xu Protocol)

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Revision as of 11:16, 25 July 2013 by Megan N McClean (talk | contribs) (Overview)
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This is the protocol used by P. Xu for fixing yeast cells for GFP fluorescence.


  • Yeast cells
  • Formaldehyde 37% (Sigma-Aldrich, #252549)
  • Phosphate buffered saline??


  • Prepare eppendorf tubes with 50μL of 37% formaldehyde, one per sample.
  • Add 450μL of culture to 50μL of formaldehyde in an eppendorf tube and mix by inversion. (The goal is to have a final concentration of formaldehyde around 3.7%. So you can adjust the cell and formaldehyde volumes accordingly, as long as you end up with 3.7% formaldehyde).
  • Incubate the tube at room temperature for 15-20 minutes.
  • Spin down ??
  • Resuspend cells in ??
  • Store samples at 4°C until you are ready to image.


Please feel free to post comments, questions, or improvements to this protocol. Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.



Ping Xu 3:12, 25 July 2013 (EDT)

or instead, discuss this protocol.