Matt Gethers

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Revision as of 21:28, 13 February 2007 by Mgethers (talk | contribs) (M13 Engineering Ideas)
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I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.

20.109 Work

M13 Engineering Ideas

Gene Modification
X Extract from gene II
II Extract gene X
V
VII Separate from gene IX
III Change the GTG to ATG Start?
VI
I Separate from genes IV and XI
XI Separate from genes I and IV
IV Separate from genes I and XI
M13 ORI 1
KanR
M13 ORI 2

Extractions, separations, etc. include ensuring that each gene has its own promoter, RBS, terminator, and any other pertinent regulatory sequences.

In addition to separating the genes, it would also be wise to develop or utilize/modify an existing system like [[ ||Biobricks]] to build the genome so that convenient restriction sites exist between the genes so they can be easily removed or replaced.