Matt Gethers

I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.

20.109 Work

• M13 Engineering Ideas

• 2.27.06 Ligation and Transformation Data

• 2.27.09 Refactoring Work

• 4.3.07_Primer_Design_Team_Purple.doc

• Research Proposal

Reagents

List of Needed Reagents:

1. Tris.SO₄
2. Dithiothreitol
3. Sorbitol
4. KP_i
5. Zymolyase 5000
6. Mannitol
7. Tris-HCl
8. Phenylmethylsulfonyl fluoride (PMSF)
9. PBS
10. Beta Mercaptoethanol
11. Reticulocyte Lysate
12. Hepes/KOH
13. ATP
14. MgCl₂
15. Phosphoenolpyruvate (PEP)
16. Pyruvate kinase
17. GTP
18. PEG 3500
19. LiAc
20. Carrier DNA (Deoxyribonucleic acid Sodium Salt Type III from Salmon Testes, Sigma D1626)
21. EDTA