Difference between revisions of "Mathies:Valves"

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[[Category:Protocol]]
 
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[[Category:Microfluidics]]
 
[[Category:Microfluidics]]
  
 
<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL!  -->
 
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[[Image:valvesassembly_1.jpg]]
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==Overview==
 
==Overview==
  
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This protocol explains how to fabricate microvalves on a glass chip with a PDMS manifold.
  
 
==Materials==
 
==Materials==
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==Contact==
 
==Contact==
*Who has experience with this protocol?
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*'''[[User:Samantha Cronier|Samantha Cronier]] 19:25, 9 April 2009 (EDT)''':
  
 
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].  
 
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].  
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[[Category:Protocol]]
 
[[Category:Protocol]]
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[[Category:Microfluidics]]
  
  

Revision as of 15:25, 9 April 2009

Valvesassembly 1.jpg



Overview

This protocol explains how to fabricate microvalves on a glass chip with a PDMS manifold.

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

  • supply 1 (i.e. tubes of a certain size? spreaders?)
  • reagent 1
  • X μL reagent 2
    • component A (reagent 2 is made up of multiple components)
    • component B
  • equipment 1
  • equipment 2

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. PubMed ID:6947258 | HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. PubMed ID:13718526 | HubMed [Jacob-JMB-1961]
  3. ISBN:0879697164 [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed | HubMed

Contact

or instead, discuss this protocol.