Difference between revisions of "Marie-Emmanuelle Kerros"

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HOW TO ... MAKE PCR REAGENTS
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[[Category:User France]]
 
 
Rutgers, October 2004
 
 
 
 
 
1- 5x Tris Boric acid EDTA buffer
 
 
 
For 2 liters:
 
- TRIS base (Boehringer Mannheim 1814273): 108 g
 
- Boric acid electrophoresis grade (FisherBiotech BP168-500): 55 g
 
- 0.5 M EDTA (pH 8.0): 40 ml (or 9.3 g Na2EDTA)
 
- adjust to pH 8.3
 
- H2O to 2 liters
 
 
 
 
 
2- EDTA 0.5 M pH = 8.0
 
- EDTA: 18.61 g
 
- NaOH: 2.0 g
 
- H2O: 80 ml
 
- dissolve on a magnetic stirrer
 
- adjust to pH = 8.0 with NaOH 10N and 1N
 
- H2O to 100 ml
 

Latest revision as of 23:58, 19 June 2008