Difference between revisions of "Marek: Freeze-down/Thaw"

From OpenWetWare
Jump to: navigation, search
(Contact)
(Contact)
Line 44: Line 44:
  
 
<!-- You can tag this protocol with various categories.  See the [[Categories]] page for more information. -->
 
<!-- You can tag this protocol with various categories.  See the [[Categories]] page for more information. -->
 +
[[Category:Mammalian cell culture]]
 +
[[Category:In vitro]]
 
[[Category:Protocol]]
 
[[Category:Protocol]]
[[Category:Mammalian cell culture]]
 
 
<!-- Move the relevant categories above this line to tag your protocol with the label
 
[[Category:In vitro]]
 
 
[[Category:In vivo]]
 
 
[[Category:In silico]]
 
 
[[Category:DNA]]
 
 
[[Category:RNA]]
 
 
[[Category:Protein]]
 
 
[[Category:Chemical]]
 
 
 
[[Category:Yeast]]
 
-->
 

Revision as of 01:48, 18 May 2007

Overview

This short protocol describes how to freeze down and thaw (or bring up) mammalian cells (e.g. HeLa, KEK293, CHO, Jurkat T cells).

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

  • supply 1 (i.e. tubes of a certain size? spreaders?)
  • reagent 1
  • X μL reagent 2
    • component A (reagent 2 is made up of multiple components)
    • component B
  • equipment 1
  • equipment 2

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. PubMed ID:6947258 | HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. PubMed ID:13718526 | HubMed [Jacob-JMB-1961]
  3. Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. ISBN:0879697164 [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed | HubMed

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.