Difference between revisions of "Manual 2ab Assembly Digest"

From OpenWetWare
Jump to: navigation, search
(New page: Add 4uL of Miniprepped DNA to 7uL of either Lefty or Righty MasterMix. <br> '''Lefty MasterMix''' (for 4uL miniprep DNA) *4uL water *1uL of NEB2 *1uL XhoI *1uL BamH1 '''Righty MasterMix'...)
 
 
Line 5: Line 5:
 
*1uL of NEB2
 
*1uL of NEB2
 
*1uL XhoI
 
*1uL XhoI
*1uL BamH1
+
*1uL ''BamH1''
  
 
'''Righty MasterMix''' (for 4uL miniprep DNA)
 
'''Righty MasterMix''' (for 4uL miniprep DNA)
Line 11: Line 11:
 
*1uL of NEB2
 
*1uL of NEB2
 
*1uL XhoI
 
*1uL XhoI
*1uL BglII <br>
+
*1uL ''BglII'' <br>
  
 
Incubate for 1 hour at 37deg C.
 
Incubate for 1 hour at 37deg C.

Latest revision as of 11:12, 11 June 2010

Add 4uL of Miniprepped DNA to 7uL of either Lefty or Righty MasterMix.

Lefty MasterMix (for 4uL miniprep DNA)

  • 4uL water
  • 1uL of NEB2
  • 1uL XhoI
  • 1uL BamH1

Righty MasterMix (for 4uL miniprep DNA)

  • 4uL water
  • 1uL of NEB2
  • 1uL XhoI
  • 1uL BglII

Incubate for 1 hour at 37deg C.