From OpenWetWare
Revision as of 12:35, 24 June 2010 by Christopher J. Zopf (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Home        Research        Lab Members        Publications        Software        Internal       

Protocol for Nucleic Acid Precipitation from Diluted Solutions

  1. Add 1/10 volume of 3M sodium acetate (or 2M sodium chloride, or 5M ammonium acetate) to DNA solution.
  2. Add Glycogen to a final 0.05-1µg/µl concentration. Use up to 1µl of Glycogen per 20µl of the solution.
  3. Add 1 volume of isopropanol (or 2.5 volumes of ethanol) to the solution. Mix gently but thoroughly. Ethanol is recommended for precipitation of smaller than 200 b/bp RNA/DNA fragments.
  4. Incubate the mixture at -20°C for up to 60min, or at -70°C for 30min.
  5. Centrifuge the mixture for 10-15min at 10,000rpm. Discard the supernatant.
  6. Rinse the pellet with cold 70% ethanol. Air-dry the pellet.
  7. Dissolve DNA in Water, nuclease-free <> (#R0581) or TE buffer.
  8. Dissolve RNA in DEPC-treated Water <> (#R0601).

Using only sodium acetate and ethanol works well - CJZ 6/24/10