Magni:Protocols/ph: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = pH adjustment for LB medium = *Before autoclaving the LB medium: ** dip pH-meter probe in the medium to m...) |
No edit summary |
||
| Line 4: | Line 4: | ||
= pH adjustment for LB medium = | = pH adjustment for LB medium = | ||
*Before autoclaving the LB medium: | *Before autoclaving the LB medium: | ||
** dip pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required); | ** dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required); | ||
** ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections! | ** ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections! | ||
** wash the pH probe with deionized water and store it in KCl 3M. | ** wash the pH probe with deionized water and store it in KCl 3M. | ||
* Autoclave the medium | * Autoclave the medium | ||
<div style="padding: 10px; width: 720px; border: 5px solid #000000;"> | |||
= pH adjustment for M9 medium = | |||
*According to the M9 medium preparation protocol, mix: | |||
**water, | |||
**glycerol and | |||
**M9 salts. | |||
**Dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required); | |||
** ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections! | |||
** wash the pH probe with deionized water and store it in KCl 3M. | |||
* Autoclave the mix in a bottle. | |||
* Equilibrate at room temperature and add the remaining ingredients (casamino acids, thiamine, CaCl2, MgSO4). | |||
</div> | </div> | ||
Revision as of 08:43, 18 April 2013
pH adjustment for LB medium
- Before autoclaving the LB medium:
- dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
- ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
- wash the pH probe with deionized water and store it in KCl 3M.
- Autoclave the medium
pH adjustment for M9 medium
- According to the M9 medium preparation protocol, mix:
- water,
- glycerol and
- M9 salts.
- Dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
- ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
- wash the pH probe with deionized water and store it in KCl 3M.
- Autoclave the mix in a bottle.
- Equilibrate at room temperature and add the remaining ingredients (casamino acids, thiamine, CaCl2, MgSO4).
