Difference between revisions of "Magni:Protocols/hplc"

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(Preparation of samples for HPLC)
 
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*Filter-sterilize (0.2 um) samples and store them in 2-ml tubes at -20°C.
 
*Filter-sterilize (0.2 um) samples and store them in 2-ml tubes at -20°C.
 
*The storage of two samples is recommended for backup reasons.
 
*The storage of two samples is recommended for backup reasons.
*At least 800 ul are required for each HPLC analysis. If this volume is not available, store at least 150 ul and analyze sample with "insert".
 
 
*To start analysis, transfer the content of a 2-ml tube into a vial, seal the cap and put the vial in the auto-sampler.
 
*To start analysis, transfer the content of a 2-ml tube into a vial, seal the cap and put the vial in the auto-sampler.
 +
*At least 800 ul are required for each HPLC analysis. If this volume is not available, store at least 150 ul and analyze sample in an "insert" inside a vial.
  
 
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Latest revision as of 07:15, 5 May 2013

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Preparation of samples for HPLC

  • Filter-sterilize (0.2 um) samples and store them in 2-ml tubes at -20°C.
  • The storage of two samples is recommended for backup reasons.
  • To start analysis, transfer the content of a 2-ml tube into a vial, seal the cap and put the vial in the auto-sampler.
  • At least 800 ul are required for each HPLC analysis. If this volume is not available, store at least 150 ul and analyze sample in an "insert" inside a vial.