Difference between revisions of "Magni:Protocols/Miniprep"

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(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = Miniprep = *Follow the NucleoSpin Plasmid kit (Macherey-Nagel) instructions, with the exception that elut...)
 
(Miniprep)
 
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<div style="padding: 10px; width: 720px; border: 5px solid #000000;">
 
<div style="padding: 10px; width: 720px; border: 5px solid #000000;">
 
= Miniprep =
 
= Miniprep =
*Follow the NucleoSpin Plasmid kit (Macherey-Nagel) instructions, with the exception that elution can be done with 30 ul of deionized water ()
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*Use the NucleoSpin Plasmid kit (Macherey-Nagel) instructions, with the following exceptions:
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** saturated bacterial culture can be centrifuged at 4000 rpm, 4degC, 10 min;
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** elution step can be done with 30 ul of Milli-Q water (from the filter of the Millipore machine).

Latest revision as of 13:51, 9 September 2012

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Miniprep

  • Use the NucleoSpin Plasmid kit (Macherey-Nagel) instructions, with the following exceptions:
    • saturated bacterial culture can be centrifuged at 4000 rpm, 4degC, 10 min;
    • elution step can be done with 30 ul of Milli-Q water (from the filter of the Millipore machine).