Difference between revisions of "Magni:Back Door"

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(E. coli strains)
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== Antibiotics ==
+
== Antibiotics, inducers and substrates ==
 
*Ampicillin - 100 mg/ml in deionized water (-20°C)
 
*Ampicillin - 100 mg/ml in deionized water (-20°C)
 
*Chloramphenicol - 34 mg/ml in ethanol (-20°C)
 
*Chloramphenicol - 34 mg/ml in ethanol (-20°C)
 
*Kanamycin - 50 mg/ml in deionized water (-20°C)
 
*Kanamycin - 50 mg/ml in deionized water (-20°C)
 
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*Gentamycin - 50 mg/ml in deionized water (-20°C)
== Inducers and substrates ==
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*G418 - 50 mg/ml in deionized water (+4°C)
*HSL (3-oxo-C6-homoserine lactone) - 2 mM in water (-20°C)
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*HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol 70% or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
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*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
 
*IPTG Ready Made - 200 mM (-20°C)
 
*IPTG Ready Made - 200 mM (-20°C)
 
*X-Gal - 40 mg/ml in DMF (-20°C)
 
*X-Gal - 40 mg/ml in DMF (-20°C)
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== ''E. coli'' strains ==
 
== ''E. coli'' strains ==
 
*BW20767 (Registry)
 
*BW20767 (Registry)
 
 
*BW23474 (CGSC)
 
*BW23474 (CGSC)
**pir-116 strain, used to propagate the R6K conditional replication origin at high copy
 
 
 
*BW25141 (CGSC)
 
*BW25141 (CGSC)
**pir+ strain, used to propagate the R6K conditional replication origin at medium copy
 
 
 
*BW25142 (CGSC)
 
*BW25142 (CGSC)
**pir-116 strain, used to propagate the R6K conditional replication origin at high copy (equivalent to BW23474)
 
 
 
*CW2553 + pJat8 (Registry)
 
*CW2553 + pJat8 (Registry)
 
 
*DB3.1 (Invitrogen)
 
*DB3.1 (Invitrogen)
**can propagate plasmids with ccdB toxin
 
 
 
*DH5alpha (Invitrogen)
 
*DH5alpha (Invitrogen)
**general purpose cloning
 
**blue/white ok
 
 
 
*KRX (Promega)
 
*KRX (Promega)
**overexpresses LacI via the lacIq mutation on the F plasmid
 
**can be used for autoinduction protocol (see Promega handbook)
 
**blue/white ok
 
 
 
*MC1061 (CGSC)
 
*MC1061 (CGSC)
**no blue/white screening
 
 
 
*MG1655 (CGSC)
 
*MG1655 (CGSC)
**K-12 sequenced strain
 
**no blue/white screening
 
 
 
*MG1655-Z1 (Univ of Washington)
 
*MG1655-Z1 (Univ of Washington)
**MG1655 with lacI- and tetR- overexpression cassettes in the genome, in the attB(lambda) locus
 
 
 
*TOP10 (Invitrogen)
 
*TOP10 (Invitrogen)
**general purpose cloning
+
*W3110 (DSMZ)
**blue/white ok
 
 
 
 
*XL1-Blue (Stratagene)
 
*XL1-Blue (Stratagene)
 
== Important glycerol stocks ==
 
  
 
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Revision as of 10:40, 28 March 2013

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Antibiotics, inducers and substrates

  • Ampicillin - 100 mg/ml in deionized water (-20°C)
  • Chloramphenicol - 34 mg/ml in ethanol (-20°C)
  • Kanamycin - 50 mg/ml in deionized water (-20°C)
  • Gentamycin - 50 mg/ml in deionized water (-20°C)
  • G418 - 50 mg/ml in deionized water (+4°C)
  • HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
  • aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
  • IPTG Ready Made - 200 mM (-20°C)
  • X-Gal - 40 mg/ml in DMF (-20°C)

E. coli strains

  • BW20767 (Registry)
  • BW23474 (CGSC)
  • BW25141 (CGSC)
  • BW25142 (CGSC)
  • CW2553 + pJat8 (Registry)
  • DB3.1 (Invitrogen)
  • DH5alpha (Invitrogen)
  • KRX (Promega)
  • MC1061 (CGSC)
  • MG1655 (CGSC)
  • MG1655-Z1 (Univ of Washington)
  • TOP10 (Invitrogen)
  • W3110 (DSMZ)
  • XL1-Blue (Stratagene)