Difference between revisions of "Magni:Back Door"

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(Antibiotics, inducers and substrates)
(E. coli strains)
 
(2 intermediate revisions by the same user not shown)
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*Gentamycin - 50 mg/ml in deionized water (-20°C)
 
*Gentamycin - 50 mg/ml in deionized water (-20°C)
 
*G418 - 50 mg/ml in deionized water (+4°C)
 
*G418 - 50 mg/ml in deionized water (+4°C)
*HSL (3-oxo-C6-homoserine lactone) - 2 mM in water (-20°C)
+
*HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
 
*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
 
*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
 
*IPTG Ready Made - 200 mM (-20°C)
 
*IPTG Ready Made - 200 mM (-20°C)
Line 15: Line 15:
  
 
== ''E. coli'' strains ==
 
== ''E. coli'' strains ==
 +
*B (DSMZ)
 +
*B/r (DSMZ)
 
*BW20767 (Registry)
 
*BW20767 (Registry)
 
*BW23474 (CGSC)
 
*BW23474 (CGSC)
 
*BW25141 (CGSC)
 
*BW25141 (CGSC)
 
*BW25142 (CGSC)
 
*BW25142 (CGSC)
 +
*C (DSMZ)
 
*CW2553 + pJat8 (Registry)
 
*CW2553 + pJat8 (Registry)
 +
*Crooks (ATCC)
 
*DB3.1 (Invitrogen)
 
*DB3.1 (Invitrogen)
 
*DH5alpha (Invitrogen)
 
*DH5alpha (Invitrogen)
Line 26: Line 30:
 
*MG1655 (CGSC)
 
*MG1655 (CGSC)
 
*MG1655-Z1 (Univ of Washington)
 
*MG1655-Z1 (Univ of Washington)
 +
*ML308 (DSMZ)
 
*TOP10 (Invitrogen)
 
*TOP10 (Invitrogen)
 +
*W (DSMZ)
 +
*W3110 (CGSC)
 
*XL1-Blue (Stratagene)
 
*XL1-Blue (Stratagene)
  
 
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Latest revision as of 01:26, 4 December 2016

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Antibiotics, inducers and substrates

  • Ampicillin - 100 mg/ml in deionized water (-20°C)
  • Chloramphenicol - 34 mg/ml in ethanol (-20°C)
  • Kanamycin - 50 mg/ml in deionized water (-20°C)
  • Gentamycin - 50 mg/ml in deionized water (-20°C)
  • G418 - 50 mg/ml in deionized water (+4°C)
  • HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
  • aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
  • IPTG Ready Made - 200 mM (-20°C)
  • X-Gal - 40 mg/ml in DMF (-20°C)

E. coli strains

  • B (DSMZ)
  • B/r (DSMZ)
  • BW20767 (Registry)
  • BW23474 (CGSC)
  • BW25141 (CGSC)
  • BW25142 (CGSC)
  • C (DSMZ)
  • CW2553 + pJat8 (Registry)
  • Crooks (ATCC)
  • DB3.1 (Invitrogen)
  • DH5alpha (Invitrogen)
  • KRX (Promega)
  • MC1061 (CGSC)
  • MG1655 (CGSC)
  • MG1655-Z1 (Univ of Washington)
  • ML308 (DSMZ)
  • TOP10 (Invitrogen)
  • W (DSMZ)
  • W3110 (CGSC)
  • XL1-Blue (Stratagene)