From OpenWetWare
Revision as of 07:38, 2 March 2009 by Jakob Suckale (talk | contribs) (external links)
Jump to: navigation, search

Recipe for 10x MOPS buffer

  • 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
  • 10.9g Sodium Acetate, 3- hydrate
  • 3.7g EDTA, sodium salt

  • add 800ml of nuclease free distilled water; mix to dissolve
  • adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
  • fill to the final volume of 1000 ml

  • filter sterilise
  • store at room temperature
  • protect from light; do not use if the solution appears yellow

Some OWW protocols which use MOPS

External links