Difference between revisions of "MOPS"

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== External links ==
== External links ==
* [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS]
* [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS]
* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8332?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=mops&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT&text_only=true CSH Protocols MOPS] [[Image:Padlock-closed.png]]

Revision as of 08:39, 2 March 2009

The other mops

MOPS is the common name for the buffering compound in MOPS buffer. MOPS stands for 3-(N-morpholino) propanesulfonic acid and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. HEPES is a chemically similar pH buffering compound.

Recipe for 10x MOPS buffer

chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid
  • 41.2 g 3-(N-morpholino) propanesulfonic acid (MOPS); MW 209.3 g/mol
  • 10.9 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is lighter with MW = 82 g/mol)
  • 3.7 g EDTA, sodium salt; MW 292.2 g/mol

  • add 800 ml of nuclease free distilled water; mix to dissolve
  • adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
  • fill to the final volume of 1000 ml

  • filter sterilise or autoclave
  • store at room temperature
  • protect from light; do not use if the solution appears yellow

Final concentration of active compounds in 10x stock

  • 400 mM MOPS (buffering)
  • 100 mM NaAc
  • 10 mM EDTA (nuclease inhibition by Mg2+ chelation)

Stability of MOPS

Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. Solution will turn yellow but this does not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [1]

Some OWW protocols which use MOPS

External links