Difference between revisions of "MES"

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2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9
 
2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9
<cite>Good66</cite><cite>Good74</cite><cite>Blanchard84</cite>.  Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.
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<cite>Good66,Good74,Blanchard84</cite>.  Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.
  
* pK<sub>a</sub> at 25C of 6.10 (5.97 at 37C)
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* pK<sub>a</sub> at 25°C of 6.10 (5.97 at 37°C)
 
* usable buffering range of 5.5 to 6.7
 
* usable buffering range of 5.5 to 6.7
 
* molecular weight 195.24
 
* molecular weight 195.24

Latest revision as of 13:05, 25 February 2009

2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9 [1, 2, 3]. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.

  • pKa at 25°C of 6.10 (5.97 at 37°C)
  • usable buffering range of 5.5 to 6.7
  • molecular weight 195.24
  • ΔpKa/ΔT = -.011

Buffers are typically 1 M, prepared by neutralizing MES with sodium hydroxide.

  1. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. PubMed ID:5942950 | HubMed [Good66]
  2. Good NE and Izawa S. Hydrogen ion buffers. Methods Enzymol. 1972;24:53-68. PubMed ID:4206745 | HubMed [Good74]
  3. Blanchard JS. Buffers for enzymes. Methods Enzymol. 1984;104:404-14. PubMed ID:6717292 | HubMed [Blanchard84]
All Medline abstracts: PubMed | HubMed and