MES: Difference between revisions
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2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9 | 2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9 | ||
<cite>Good66 | <cite>Good66,Good74,Blanchard84</cite>. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. | ||
* pK<sub>a</sub> at | * pK<sub>a</sub> at 25°C of 6.10 (5.97 at 37°C) | ||
* usable buffering range of 5.5 to 6.7 | * usable buffering range of 5.5 to 6.7 | ||
* molecular weight 195.24 | * molecular weight 195.24 | ||
| Line 14: | Line 14: | ||
# Blanchard84 pmid=6717292 | # Blanchard84 pmid=6717292 | ||
</biblio> | </biblio> | ||
[[Category:Material]] and [[Category:Buffers]] | |||
Latest revision as of 14:05, 25 February 2009
2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9 [1, 2, 3]. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.
- pKa at 25°C of 6.10 (5.97 at 37°C)
- usable buffering range of 5.5 to 6.7
- molecular weight 195.24
- ΔpKa/ΔT = -.011
Buffers are typically 1 M, prepared by neutralizing MES with sodium hydroxide.
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. DOI:10.1021/bi00866a011 |
- Good NE and Izawa S. Hydrogen ion buffers. Methods Enzymol. 1972;24:53-68. DOI:10.1016/0076-6879(72)24054-x |
- Blanchard JS. Buffers for enzymes. Methods Enzymol. 1984;104:404-14. DOI:10.1016/s0076-6879(84)04107-0 |
and
