Difference between revisions of "MES"

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# Blanchard84 pmid=6717292
 
# Blanchard84 pmid=6717292
 
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[[Category:Material]] and [[Category:Buffers]]

Revision as of 13:32, 11 March 2008

2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9 [1][2][3]. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.

  • pKa at 25C of 6.10 (5.97 at 37C)
  • usable buffering range of 5.5 to 6.7
  • molecular weight 195.24
  • ΔpKa/ΔT = -.011

Buffers are typically 1 M, prepared by neutralizing MES with sodium hydroxide.

  1. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. PubMed ID:5942950 | HubMed [Good66]
  2. Good NE and Izawa S. Hydrogen ion buffers. Methods Enzymol. 1972;24:53-68. PubMed ID:4206745 | HubMed [Good74]
  3. Blanchard JS. Buffers for enzymes. Methods Enzymol. 1984;104:404-14. PubMed ID:6717292 | HubMed [Blanchard84]
All Medline abstracts: PubMed | HubMed and