Difference between revisions of "MEG"

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(Molecules of Equivalent GFP (MEG))
 
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==Molecules of Equivalent GFP (MEG)==
 
==Molecules of Equivalent GFP (MEG)==
  
Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot.
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[[Image:Cconboy-Flow Calibration.png|200px]]
  
[[Image:FP-0852-2 MEG calibration.pdf|left|600px]]
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[Image:Cconboy-Flow Calibration.png|200px]
  
 
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Fig.1. Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quantitative western blot. The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted.
The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted.
 
  
 
===Standard curve===
 
===Standard curve===

Latest revision as of 18:36, 29 May 2013

Molecules of Equivalent GFP (MEG)

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[Image:Cconboy-Flow Calibration.png|200px]

Fig.1. Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quantitative western blot. The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted.

Standard curve

[Fluorescence intensity] = 0.0025*[GFP copies/cell]-14.416

R^2 = 0.9684

FP-0852-2 MEG

From the standard curve above, FP-0852-2 beads represent ~370,000 MEG (+/-40%)

Reference

This work was done by Caitlin Conboy and Jennifer Braff. Thorough documentation to come...