Lum

Procedures

Quantification

1. Instument Qubit Fluoromete
2. Setting Broad Range
   • Use Broad Range Standards in BR kit
3. Turn lights off, reagents are light sensitive
4. Prepare Working Solution
   • 199 µl Quant iT Buffer : 1 µl Quant iT Reagent
   • 199:1 for every sample needed (ex.10 samples will be 2000 µl total)
5. Prepare Samples
6. *190 µl working solution + 10 µl standard 1 solution
7. *190 µl working solution + 10 µl standard 2 solution
8. *195 µl working solution + 5 µl of DNA
9. Vortex for 2-3 minutes
10. Incubate 15 minutes
11. Follow On Screen Directions