Difference between revisions of "Lum"

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(New page: ==Procedures== ===Quantification=== # '''Instument''' Qubit Fluoromete # '''Setting''' Broad Range #* Use Broad Range Standards in BR kit # Turn lights off, reagents are light sensitive #...)
 
(Procedures)
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==Procedures==
+
==Research interests==
===Quantification===
 
  
# '''Instument''' Qubit Fluoromete
+
# [Microbial Community Analysis]: Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant “lupines chamissonis”
# '''Setting''' Broad Range
+
# [Tommy]
#* Use Broad Range Standards in BR kit
+
# [Jodie]
# Turn lights off, reagents are light sensitive
+
# [Elise]
# Prepare '''Working Solution'''
+
# [Amanda]
#* 199 µl '''Quant iT Buffer''' : 1 µl '''Quant iT Reagent'''
 
#*199:1 for every sample needed (ex.10 samples will be 2000 µl total)
 
# Prepare Samples
 
# *190 µl working solution + 10 µl standard 1 solution
 
# *190 µl working solution + 10 µl standard 2 solution
 
# *195 µl working solution + 5 µl of DNA
 
# Vortex for 2-3 minutes
 
# Incubate 15 minutes
 
# Follow On Screen Directions
 

Revision as of 22:02, 13 September 2010

Research interests

  1. [Microbial Community Analysis]: Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant “lupines chamissonis”
  2. [Tommy]
  3. [Jodie]
  4. [Elise]
  5. [Amanda]