Difference between revisions of "Loading dye"

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==Purpose==
 
==Purpose==
Loading dye is mixed with DNA samples for use in agarose gel electrophoresis.  It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).
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Loading dye is mixed with samples for use in gel electrophoresis.  It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).
  
==Procurement==
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==Types of loading dyes==
Here's a sample recipe for loading dye.
 
  
*Ficoll 400 (in 68-558F)
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[[Agarose gel loading dye]]
*Deionized water
 
*Orange G dye
 
 
 
Dissolve 1.5 g of Ficoll in 10 mL of deionized water.  Add very small amounts of Orange G dye such that the loading dye is dark orange.  Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.
 
 
 
===Specific recipes===
 
*[[Knight:Loading dye]]
 
 
 
==Use==
 
*Orange G: generally runs very fast (<100 bp)
 
*Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose)
 
*Xylene cyanol: blue, runs at ~4kb
 

Latest revision as of 11:53, 25 August 2006

Purpose

Loading dye is mixed with samples for use in gel electrophoresis. It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).

Types of loading dyes

Agarose gel loading dye