Difference between revisions of "Loading dye"

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==Loading dye==
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==Purpose==
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Loading dye is mixed with samples for use in gel electrophoresis.  It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).
  
*Ficoll 400
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==Types of loading dyes==
*Deionized water
 
*Orange G dye
 
  
Dissolve 1.5 g of Ficoll in 10 mL of deionized water.  Add very small amounts of Orange G dye such that the loading dye is dark orange.  Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.
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[[Agarose gel loading dye]]

Latest revision as of 12:53, 25 August 2006

Purpose

Loading dye is mixed with samples for use in gel electrophoresis. It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).

Types of loading dyes

Agarose gel loading dye