Difference between revisions of "Loading dye"

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==Loading dye==
 
==Loading dye==
  
*Ficoll 400
+
*Ficoll 400 (in 68-558F)
 
*Deionized water
 
*Deionized water
 
*Orange G dye
 
*Orange G dye
  
 
Dissolve 1.5 g of Ficoll in 10 mL of deionized water.  Add very small amounts of Orange G dye such that the loading dye is dark orange.  Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.
 
Dissolve 1.5 g of Ficoll in 10 mL of deionized water.  Add very small amounts of Orange G dye such that the loading dye is dark orange.  Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.

Revision as of 08:53, 10 August 2005

Loading dye

  • Ficoll 400 (in 68-558F)
  • Deionized water
  • Orange G dye

Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.