Difference between revisions of "Lidstrom: Agarose Gel Electrophoresis"

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** If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.  
 
** If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.  
 
* Load sample  
 
* Load sample  
 +
** 1.7 uL of a PCR product is usually plenty
 
* Load ladder
 
* Load ladder
 
** If using MassRuler, 4 uL is good for skinny lanes.  
 
** If using MassRuler, 4 uL is good for skinny lanes.  
* Run @ 100 V for 20 min and check the gel.
+
* Run @ 110 V for 20 min and check the gel.
 +
** 40 min is pretty safe for 0.7 - 1% agarose gels

Revision as of 19:51, 20 February 2013

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Starting with an empty box

  • Fill with TBE until it covers the gel holding tray.
  • Add 40 uL of ethidium bromide if your gels don't already have it within
    • If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.
  • Load sample
    • 1.7 uL of a PCR product is usually plenty
  • Load ladder
    • If using MassRuler, 4 uL is good for skinny lanes.
  • Run @ 110 V for 20 min and check the gel.
    • 40 min is pretty safe for 0.7 - 1% agarose gels