Difference between revisions of "Lidstrom: Agarose Gel Electrophoresis"

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== Starting with an empty box ==
 
== Starting with an empty box ==
 
* Fill with TBE until it covers the gel holding tray.
 
* Fill with TBE until it covers the gel holding tray.
* Add 40? uL of ethidium bromide if your gels don't already have it within
+
* Add 40 uL of ethidium bromide if your gels don't already have it within
 
** If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.  
 
** If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.  
 
* Load sample  
 
* Load sample  

Revision as of 18:19, 20 February 2013

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Starting with an empty box

  • Fill with TBE until it covers the gel holding tray.
  • Add 40 uL of ethidium bromide if your gels don't already have it within
    • If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.
  • Load sample
  • Load ladder
    • If using MassRuler, 4 uL is good for skinny lanes.
  • Run @ 100 V for 20 min and check the gel.