From OpenWetWare
Revision as of 08:01, 13 June 2013 by Janet B. Matsen (talk | contribs) (Machines available as of 6/2013)
Jump to: navigation, search

Back to Protocols

Machines available as of 6/2013

Thermocycler location Appearance Brand Capabilities Manual Use restrictions
Janet's bench black DNA Engine: DYAD small block doesn't do gradient; large block does n/a none
Amanda's window white & new Eppendorf: Mastercycler gradient gradient, but see manual b/c it is weird Mastercycler Gradient none
Frances' window black & old DNA engine no gradient n/a none
Nate's Bench ## ## ## ## none?
Alexey's Bench old beige BioRad: S1000 2 gradient blocks ## none
Ceci's window white, small Eppendorf: ??  ??  ?? none?

Specific Tips for Individual Machines


What makes for a good thermocycler?


  • gradient block(s)
  • multiple blocks on one machine
    • reduces the need to enter programs into many machines
  • allow for saving of protocols in folders
    • different researchers can have different folders, preventing the accumulation of a huge list of protocols, and others accidentally writing over other peoples' protocols


  • intuitive menu
    • NOT true of eppendorf systems!
    • BioRad system is fantastic
  • Show what happens to each well for a given gradient setting *while* the protocol is being edited
    • Not true of white eppendorf Mastercycler gradient

Bonus Points

  • ability to recall past thermocycling programs used
    • occasionally useful for troubleshooting failed reactions
  • Optional window that shows how long until a protocol is compete
    • Marina's BioRad system's screen goes black with big light up numbers indicating how long until it is complete. It can be seen from several benches away. (This machine is no longer here.)