Lidstrom:Thermocyclers: Difference between revisions
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| Nate's Bench || ## || ## || ## || ## || none? | | Nate's Bench || ## || ## || ## || ## || none? | ||
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| Alexey's Bench || | | Alexey's Bench || old beige || BioRad: S1000 || 2 gradient blocks || ## || none | ||
|- | |- | ||
| Ceci's window || white, small || Eppendorf: ?? || ?? || ?? || none? | | Ceci's window || white, small || Eppendorf: ?? || ?? || ?? || none? | ||
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== Specific Tips for Individual Machines == | |||
=== | |||
== What makes for a good thermocycler? == | == What makes for a good thermocycler? == |
Revision as of 09:01, 13 June 2013
Back to Protocols
Machines available as of 6/2013
Thermocycler location | Appearance | Brand | Capabilities | Manual | Use restrictions |
Janet's bench | black | DNA Engine: DYAD | small block doesn't do gradient; large block does | n/a | none |
Amanda's window | white & new | Eppendorf: Mastercycler gradient | gradient, but see manual b/c it is weird | Mastercycler Gradient | none |
Frances' window | black & old | DNA engine | no gradient | n/a | none |
Nate's Bench | ## | ## | ## | ## | none? |
Alexey's Bench | old beige | BioRad: S1000 | 2 gradient blocks | ## | none |
Ceci's window | white, small | Eppendorf: ?? | ?? | ?? | none? |
Specific Tips for Individual Machines
=
What makes for a good thermocycler?
Functions
- gradient block(s)
- multiple blocks on one machine
- reduces the need to enter programs into many machines
- allow for saving of protocols in folders
- different researchers can have different folders, preventing the accumulation of a huge list of protocols, and others accidentally writing over other peoples' protocols
Interface
- intuitive menu
- NOT true of eppendorf systems!
- BioRad system is fantastic
- Show what happens to each well for a given gradient setting *while* the protocol is being edited
- Not true of white eppendorf Mastercycler gradient
Bonus Points
- ability to recall past thermocycling programs used
- occasionally useful for troubleshooting failed reactions
- Optional window that shows how long until a protocol is compete
- Marina's BioRad system's screen goes black with big light up numbers indicating how long until it is complete. It can be seen from several benches away. (This machine is no longer here.)